Temperature-Sensitive Mutants of Influenza Virus. XIII. Evaluation of Influenza A/Hong Kong/68 and A/Udom/72 ts and Wild-Type Viruses in Tracheal Organ Culture at Permissive and Restrictive Temperatures
Identifieur interne : 002A49 ( Main/Exploration ); précédent : 002A48; suivant : 002A50Temperature-Sensitive Mutants of Influenza Virus. XIII. Evaluation of Influenza A/Hong Kong/68 and A/Udom/72 ts and Wild-Type Viruses in Tracheal Organ Culture at Permissive and Restrictive Temperatures
Auteurs : Steven R. Mostow [États-Unis] ; Susan Flatauer [États-Unis] ; Marilyn Paler [États-Unis] ; Brian R. Murphy [États-Unis]Source :
- Journal of Infectious Diseases [ 0022-1899 ] ; 1977.
Abstract
For development of an in vitro assay of attenuation for virus vaccine candidates, tracheal ring organ cultures from young ferrets were infected with influenza A/Hong Kong/68 and A/Udorn/72 temperature-sensitive (ts) recombinant and wild-type viruses and observed for 21 days at permissive (33 C) and restrictive (37 C) temperatures for effects on ciliated epithelium and viral replication. Results were compared with the known effects of these viruses in humans. Viruses that damaged ferret cilia and grew to high titers at 37 C were insufficiently attenuated for humans, and viruses that failed to damage cilia and grew to low titers at 33 C were excessively attenuated for humans. This system did not completely differentiate the relative virulence of A/Hong Kong/68 ts recombinants for humans but was successful for the A/Udorn/72 ts recombinants. This organ culture system has adjunctive usefulness for detection of most insufficiently attenuated influenza A vaccine candidates and for identification of excessively attenuated candidates that might contain non-ts lesions.
Url:
DOI: 10.1093/infdis/136.1.1
Affiliations:
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XIII. Evaluation of Influenza A/Hong Kong/68 and A/Udom/72 <hi rend="italic">ts</hi> and Wild-Type Viruses in Tracheal Organ Culture at Permissive and Restrictive Temperatures</title><author><name sortKey="Mostow, Steven R" sort="Mostow, Steven R" uniqKey="Mostow S" first="Steven R." last="Mostow">Steven R. Mostow</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Department of Medicine, Cleveland Metropolitan General Hospital, Cleveland, Ohio, Bethesda</wicri:cityArea></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Colorado</region></placeName><wicri:cityArea>Please address requests for reprints to Dr. Steven R. Mostow, Division of Infectious Diseases, Denver Veterans Administration Hospital, Denver</wicri:cityArea></affiliation></author><author><name sortKey="Flatauer, Susan" sort="Flatauer, Susan" uniqKey="Flatauer S" first="Susan" last="Flatauer">Susan Flatauer</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Department of Medicine, Cleveland Metropolitan General Hospital, Cleveland, Ohio, Bethesda</wicri:cityArea></affiliation></author><author><name sortKey="Paler, Marilyn" sort="Paler, Marilyn" uniqKey="Paler M" first="Marilyn" last="Paler">Marilyn Paler</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Department of Medicine, Cleveland Metropolitan General Hospital, Cleveland, Ohio, Bethesda</wicri:cityArea></affiliation></author><author><name sortKey="Murphy, Brian R" sort="Murphy, Brian R" uniqKey="Murphy B" first="Brian R." last="Murphy">Brian R. Murphy</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Department of Medicine, Cleveland Metropolitan General Hospital, Cleveland, Ohio, Bethesda</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Infectious Diseases</title><title level="j" type="abbrev">J Infect Dis.</title><idno type="ISSN">0022-1899</idno><idno type="eISSN">1537-6613</idno><imprint><publisher>The University of Chicago Press</publisher><date when="1977-07">1977</date><biblScope unit="vol">136</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="1">1</biblScope><biblScope unit="page" to="6">6</biblScope></imprint><idno type="ISSN">0022-1899</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-1899</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">For development of an in vitro assay of attenuation for virus vaccine candidates, tracheal ring organ cultures from young ferrets were infected with influenza A/Hong Kong/68 and A/Udorn/72 temperature-sensitive (ts) recombinant and wild-type viruses and observed for 21 days at permissive (33 C) and restrictive (37 C) temperatures for effects on ciliated epithelium and viral replication. Results were compared with the known effects of these viruses in humans. Viruses that damaged ferret cilia and grew to high titers at 37 C were insufficiently attenuated for humans, and viruses that failed to damage cilia and grew to low titers at 33 C were excessively attenuated for humans. This system did not completely differentiate the relative virulence of A/Hong Kong/68 ts recombinants for humans but was successful for the A/Udorn/72 ts recombinants. This organ culture system has adjunctive usefulness for detection of most insufficiently attenuated influenza A vaccine candidates and for identification of excessively attenuated candidates that might contain non-ts lesions.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Colorado</li><li>Maryland</li></region></list><tree><country name="États-Unis"><region name="Maryland"><name sortKey="Mostow, Steven R" sort="Mostow, Steven R" uniqKey="Mostow S" first="Steven R." last="Mostow">Steven R. Mostow</name></region><name sortKey="Flatauer, Susan" sort="Flatauer, Susan" uniqKey="Flatauer S" first="Susan" last="Flatauer">Susan Flatauer</name><name sortKey="Mostow, Steven R" sort="Mostow, Steven R" uniqKey="Mostow S" first="Steven R." last="Mostow">Steven R. Mostow</name><name sortKey="Murphy, Brian R" sort="Murphy, Brian R" uniqKey="Murphy B" first="Brian R." last="Murphy">Brian R. Murphy</name><name sortKey="Paler, Marilyn" sort="Paler, Marilyn" uniqKey="Paler M" first="Marilyn" last="Paler">Marilyn Paler</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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